Production of Germfree Mice by Embryo Transfer.
نویسندگان
چکیده
منابع مشابه
production of cloned mice by nuclear transfer of cumulus cells
background: over the past several years, mammals have been successfully cloned by either the splitting of an early stage embryo or nuclear transfer of adult somatic cells (nt) into oocytes. although it has been 15 years since the generation of the first cloned mammals from somatic cells by nt, the success rate for producing live offspring by this technique is low regardless of the cell type and...
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In embryo transfer experiments in mice, pseudopregnant females as recipients are prepared by sterile mating with vasectomized males. Because only females at the proestrus stage accept males, such females are selected from a stock of animals based on the appearance of their external genital tract. Therefore, the efficiency of preparing pseudopregnant females largely depends on the size of female...
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Background: For several years, mammalian cloning by splitting an early embryo or nuclear transfer into oocytes method has been successfully performed. Cloning is now also possible using adult somatic cells. Although it has now been 15 years since the first cloned mammals were generated from somatic cells using nuclear transfer (NT), the success rate for producing live offspring by cloning is lo...
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The DBA/2J mouse strain is a standard laboratory strain that is widely used for biomedical research. This strain, however, suffers from poor reproductive performance. In addition, the conditions for reliable embryo transfer (ET) of this strain have not been elucidated. The intention of this study was to determine the optimal number of embryos for transfer that allow the effective production of ...
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The general capacity of germfree mouse spleen cells to produce interferon in vitro in response to various stimuli was investigated. The interferon response of germfree mouse spleen cells in vitro, when compared with that of the conventionals, appears to be lower to some inducers. Interferon production in vitro stimulated by hemagglutinating virus of Japan (HVJ) or BHK-HVJ cells (BHK cells persi...
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ژورنال
عنوان ژورنال: Experimental Animals
سال: 1999
ISSN: 1341-1357,1881-7122
DOI: 10.1538/expanim.48.59